Wow... man I haven't thought about loading dos high, etc for a very long time.
Dos had some major limitations, and DOS by itself could not handle any more than 1 MB of ram. It called the first 640K Conventional memory, and anything above 640K "high memory". The high memory was managed by a driver called HiMem.sys (which is loaded in the config.sys file at boot time)
Once systems with more than 1 meg of RAM were available, additional drivers had to be loaded. In DOS terms any ram memory above 1meg was called "extended memory" and was managed by a driver called Emm386.
You'll want to edit the config.sys file on your floppy to see what is loading. You should see
device=a:\path\himem.sys (path may be diffrent ex c:\himem.sys)
Device=A:\emm386.exe (path may be different)
dos=high,umb
if you wanted to load your mouse driver automatically, you'd put that in the autoexec.bat file. Syntax would probably look something like this
C:\mouse\mouse.com
or to load it in high memory
LH C:\mouse\mouse.com
Some references that helped me rember this stuff.
http://www.geocities.com/politalk/dos/memory.htm
And from classic PC games (which is written a little more simply)
http://www.classicpcgames.com/?p=faqdosmemory
Man, I am so glad we don't have to deal with that crap anymore.. windows really makes life easy!!